What are the dilution methods for antibodies? -Huaqiang Electronic Network

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Our company specializes in producing high-quality antibodies that specifically bind to their corresponding antigens. Different classes of immunoglobulins can interact with various cell types, produce distinct immune responses, and play crucial roles in the immune system. Our antibodies are highly specific, and we use affinity purification methods to ensure accurate experimental results.

We maintain strict quality control over all our products and offer multiple dilution methods to help you adjust antibody concentrations according to your needs. Whether you're working on small-scale experiments or large-scale production, we can meet your requirements for product type, packaging, and purity.

Dilution Methods:

1. Method 1: Before the experiment, reconstitute the lyophilized powder antibody with sterile distilled water (or PBS), then divide the solution into 5–10 portions (e.g., 20 µL × 5 or 10 µL × 10) and store at -20°C. Use one portion at a time to avoid repeated freezing.
2. Method 2: Alternatively, you can dilute the lyophilized antibody with sterile water (50 µL), add 50% glycerol, and store it at -20°C. Take only the amount needed when using.
3. Method 3: Perform a pre-test with several dilutions (e.g., 1:100, 1:200, 1:400). You can also test background levels to determine the optimal dilution ratio before proceeding with the main experiment.

Paraffin vs. Frozen Sections:

When choosing between paraffin and frozen sections, consider the antigen stability. Paraffin sections may destroy antigenicity due to high-temperature processing, while frozen sections better preserve immunological activity without requiring antigen retrieval. However, frozen sections can lead to ice crystal formation, which might damage cell structures. They are also thicker than paraffin sections and less visually appealing.

If the primary antibody's catalog specifies "frozen section only," you should not use paraffin. If both options are listed, you have more flexibility. Paraffin sections are ideal for maintaining tissue morphology and long-term storage, while frozen sections are preferred for in situ hybridization due to better RNA preservation.

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