What are the dilution methods for antibodies? -Huaqiang Electronic Network

Our company specializes in high-specificity antibodies that are designed to bind specifically to their corresponding antigens. Different classes of immunoglobulins can interact with various cell types, generate distinct immune responses, and contribute to the body's defense mechanisms. The antibodies we offer are affinity-purified, ensuring greater accuracy in experimental results. We maintain strict quality control and provide multiple dilution methods to help you adjust antibody concentration according to your needs. Whether you're conducting small-scale experiments or large-scale production, our products are tailored to meet your specific requirements in terms of type, packaging, and purity. Here are some recommended dilution methods: **Method 1**: Before the experiment, reconstitute the lyophilized antibody powder with sterile distilled water (or PBS) and divide the solution into 5–10 aliquots (e.g., 20 μL x 5 or 10 μL x 10), then store at -20°C. Use one aliquot at a time to avoid repeated freezing. **Method 2**: You can also prepare a stock solution by dissolving the lyophilized antibody in 50 μL of sterile water (or PBS). Add 50% glycerol and store at -20°C. Take only what you need for each use. **Method 3**: Perform a pre-test with different dilutions, such as 1:100, 1:200, 1:400, and even background checks, to determine the optimal dilution ratio before starting the main experiment. When preparing the working solution, always use an appropriate antibody diluent. **Paraffin vs. Frozen Sections**: - Frozen sections do not require paraffin embedding, which helps preserve antigenicity better than paraffin sections. However, they may lead to ice crystal formation, potentially damaging cell structure. - Paraffin sections maintain better tissue morphology and are easier to store at room temperature. They are ideal for in situ hybridization due to better penetration of probes. - Always check the manufacturer’s instructions when purchasing primary antibodies. Some antibodies are suitable only for frozen sections, while others can be used with both. **Recommended Products**:

bs-15496R	ALDH3A1 aldehyde dehydrogenase 3 family, member A1 antibody
bs-12963R	Phospho-alphaB-Crystallin (Ser59) antibody
bs-12368R	ACTG2 gamma-2 actin antibody
bs-12392R	AES gp130 GAM antibody
bs-12414R	AMHR2 anti-Müllerian hormone receptor antibody
bs-12415R	ACTR1A activin receptor antibody
bs-12416R	ACTR2 2A activin receptor antibody
bs-12417R	Activin Receptor Type IIB antibody
bs-15163R	C2orf88 chromosome 88 open reading frame antibody
bs-7144R	ARHGEF18 G protein-coupled receptor antibody
bs-3763R	Activin A Receptor Type IB 1B antibody
bs-3751R	ARAF24 androgen receptor-associated protein 24 antibody
bs-0347R	Amyloid Precursor Protein (C-terminus) antibody
bs-3920R	ADCY2 adenylate cyclase antibody
bs-5037R	APOC3 apolipoprotein C3 antibody
bs-5038R	Apolipoprotein E4 antibody
bs-3830R	AMBRA1 autophagy-related protein 1 antibody
bs-5200R	Phospho-ATG4C (Ser177) antibody
bs-5205R	Phospho-ATG4C (Ser398) antibody
bs-3007R	Phospho-ASK1 (Ser966) antibody
bs-3008R	Phospho-ATXN1 (Ser775) antibody
bs-2732R	ATXN1/Ataxin-1 antibody
bs-3028R	Phospho-Amyloid Precursor Protein (Thr668) antibody
bs-3029R	Phospho-ASK1 (Ser967) antibody
bs-3031R	Phospho-ASK1 (Thr845) antibody
bs-3033R	Phospho-ATF2 (Thr69/71) antibody
bs-3034R	Phospho-ATP1A1 (Tyr10) antibody
bs-3035R	Phospho-ATP1A1 (Ser16) antibody
bs-2745R	Acetyl-CoA Carboxylase antibody
bs-3036R	Phospho-Acetyl-CoA Carboxylase alpha (Ser78) antibody
bs-3038R	Phospho-Ack1 (Tyr859+860) antibody
bs-3025R	Phospho-AMPK alpha-1 (Ser485+Ser491) antibody
bs-3026R	Phospho-AMPK beta1 (Ser108) antibody
bs-3022R	Phospho-ALK (Tyr1604) antibody
bs-2272R	Phospho-ATM (Ser1981) antibody
bs-3037R	Phospho-ATP citrate lyase (Ser455) antibody
bs-3030R	Phospho-ATR (Ser428) antibody
bs-7960R	AMN1 nuclear relocalization and spindle checkpoint protein antibody
bs-7957R	ALG11 asparagine-linked glycosylation 11 antibody
bs-3777R	ASF1A cell senescence-associated protein antibody
bs-8457R	AGPHD1 domain antibody
bs-4119R	ASGPR1 asialoglycoprotein receptor antibody
bs-9100R	ARSF aryl sulfatase F antibody
bs-6980R	AKAP5A kinase anchoring protein 5 antibody
bs-4807R	ATXN3L spinocerebellar ataxia 3 protein antibody
bs-9297R	ASTE1 antibody
bs-5212R	Phospho-APG4B (Ser309) antibody
bs-9790R	AFAP4B actin-related protein 1 antibody
bs-4235R	ADORA1 adenosine A1A receptor antibody
bs-4231R	ARSB arylsulfatase B antibody
bs-4234R	ABAT gamma-aminobutyric acid transaminase antibody
bs-6513R	A2BP1 ataxia protein 2 binding protein 1 antibody
bs-4236R	ADAM17 tumor necrosis factor alpha converting enzyme